Objective:

This Protocol aims to establish a standard Protocol for the sampling of Anemones for the Microbiome Manipulation Experiment , which we will standardize across timepoints.

Broader Context:

The samples taken today (SAM_053-SAM_079) will serve as Timepoint 1 for the initial stability time series experiment on which we will base our manipulations. Our aim is to monitor over-all stability, as well as track changes to population based one new lab conditions.

Protocol

Protocol followed the same procedure as the experiment on Sept. 21st with the following modification. The samples were run on the bead beater at 8m/s for two rounds of 60sec with a 5 minute pause interval between. In addition, for water sampling the procedure for twater from tanks remained the same. However for sampling the ASW from the source 1L was filterd throu a 0.22 micron filter by pushing 60ml through at a time with a sterile luerlock syringe. Filter ends were sealed with parafilm before freezing thr filter whole at -80 with the other samples.